Journal: International Journal of Molecular Sciences

Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

doi: 10.3390/ijms251810125

Figure Lengend Snippet: Differentially expressed miRNAs with significant variances under high-glucose and hypoxic environment.

Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

doi: 10.3390/ijms251810125

Figure Lengend Snippet: Differential expression of miRNAs in HaCaT cells under high-glucose/hypoxia versus normal conditions. ( A ) Heat map and cluster dendrogram of 143 differentially expressed miRNAs. Columns represent miRNA expression at 24 h and 48 h under normal (24 hr1 and 48 hr1) and high-glucose/hypoxia (24 hr4 and 48 hr4) conditions. ( B ) Heat map and cluster dendrogram of 11 miRNAs with the highest changes on the expression levels at 24 and 48 h post-wounding (labeled with red asterisks in ( A )). This heat map shows miRNA changes after the normalization of the high-glucose/hypoxia condition over the normal condition. Subsequent qRT-PCR verification on these miRNAs is shown in .

Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

Techniques: Quantitative Proteomics, Expressing, Labeling, Quantitative RT-PCR

Journal: International Journal of Molecular Sciences

Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

doi: 10.3390/ijms251810125

Figure Lengend Snippet: Verification of the expression patterns of the cDNA microarray by qRT-PCR. ( A ) Differentially expressed miRNAs at 24 h post-wounding. ( B ) Differentially expressed miRNAs at 48 h post-wounding. A total of 11 miRNAs were selected for further verification by qRT-PCR in this study. In the figures, the relative miRNA expression levels are all expressed on a log2 scale, with data from the cDNA microarray shown in blue, and data from three independent experiments of qRT-PCR shown in red.

Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

Techniques: Expressing, Microarray, Quantitative RT-PCR

Journal: International Journal of Molecular Sciences

Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

doi: 10.3390/ijms251810125

Figure Lengend Snippet: Effects of miR-3138 mimic and miR-3679-5p inhibitor transfection on keratinocyte migration. The present experiments were performed under normal ( A – C ) and high-glucose/hypoxic conditions ( D – F ). ( A , D ) Relative miR-3138 and miR-3679-5p levels post-transfection. ( B , E ) Representative images of keratinocyte migration after the transfection with miRNA mimic and inhibitor. ( C , F ) Average wound closure percentages from three independent experiments after the transfection with miRNA mimic and inhibitor. Different letters labeled at the top of columns indicate statistically significant differences ( p < 0.05).

Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

Techniques: Transfection, Migration, Labeling

Journal: International Journal of Molecular Sciences

Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

doi: 10.3390/ijms251810125

Figure Lengend Snippet: Bioinformatic prediction of the target genes for miR-3138 ( A ) and miR-3679-5p ( B ). Venn diagrams show overlapped target numbers predicted by TargetScan (red), miRDB (green), and DIANA (blue) databases. The pairing patterns between the sequences of target gene 3’UTR and miRNA are shown below each diagram.

Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: MicroRNA Signature in an In Vitro Keratinocyte Model of Diabetic Wound Healing

doi: 10.3390/ijms251810125

Figure Lengend Snippet: Primers used in this study.

Article Snippet: Small RNAs (<200 nucleotides) were enriched using cutoff concentrators (Sartorius, Gottingen, Germany). miRNAs were labeled with Cy5 dye (ULS ® microRNA Labeling Kit, Kreatech Diagnostics, Amsterdam, Netherlands) and hybridized to human miRNA OneArray ® 4.1 microarray chips (Phalanx Biotech Group, Hsinchu, Taiwan).

Techniques: Sequencing